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mdlx enhancer element  (Addgene inc)


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    Addgene inc mdlx enhancer element
    A) Representative slice <t>showing</t> <t>mDlx-GFP</t> viral vector expression in the prelimbic mPFC. Co-labeling of mDlx-GFP and GAD67 demonstrates selectivity of the mDlx enhancer element for interneurons, as previously observed [20]. B) Simplified cartoon of cortical cell architecture, demonstrating approximate innervation profiles of deep-layer Fast-Spiking (FS; green) and Martinotti (MART; dark blue) interneurons. X94 cells (light blue) are somatostatin-positive cells generally located in Layer IV, and neurogliaform cells (NGF; yellow), as well as VIP-positive cells, are found largely in the superficial layers. C) For the present study, we confined our analyses to prototypical FS and Martinotti cells according to criteria outlined in the methods. Proportions of these cells were approximately equal in males and females. D-E) These neuron subtypes were readily discriminable by both spiking and input resistance. F) Single cell real-time qPCR revealed heterogeneity of Parv, Som, and Hcn1 markers in both FS and Martinotti cells, and thus did not prove especially valuable for subtype discrimination. Passive membrane properties of FS and Martinotti neurons from water-exposed animals are also shown. G) Representative traces depict fundamental differences in FS (left) and Martinotti (right) neuron functional characteristics, as well as the effect of application of the HCN channel antagonist ZD-7288 (red; 50 μM).
    Mdlx Enhancer Element, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mdlx enhancer element/product/Addgene inc
    Average 95 stars, based on 46 article reviews
    mdlx enhancer element - by Bioz Stars, 2026-04
    95/100 stars

    Images

    1) Product Images from "Chronic Ethanol Exposure Alters Prelimbic Prefrontal Cortical Fast-Spiking and Martinotti Interneuron Function With Differential Sex Specificity in Rat Brain"

    Article Title: Chronic Ethanol Exposure Alters Prelimbic Prefrontal Cortical Fast-Spiking and Martinotti Interneuron Function With Differential Sex Specificity in Rat Brain

    Journal: Neuropharmacology

    doi: 10.1016/j.neuropharm.2019.107805

    A) Representative slice showing mDlx-GFP viral vector expression in the prelimbic mPFC. Co-labeling of mDlx-GFP and GAD67 demonstrates selectivity of the mDlx enhancer element for interneurons, as previously observed [20]. B) Simplified cartoon of cortical cell architecture, demonstrating approximate innervation profiles of deep-layer Fast-Spiking (FS; green) and Martinotti (MART; dark blue) interneurons. X94 cells (light blue) are somatostatin-positive cells generally located in Layer IV, and neurogliaform cells (NGF; yellow), as well as VIP-positive cells, are found largely in the superficial layers. C) For the present study, we confined our analyses to prototypical FS and Martinotti cells according to criteria outlined in the methods. Proportions of these cells were approximately equal in males and females. D-E) These neuron subtypes were readily discriminable by both spiking and input resistance. F) Single cell real-time qPCR revealed heterogeneity of Parv, Som, and Hcn1 markers in both FS and Martinotti cells, and thus did not prove especially valuable for subtype discrimination. Passive membrane properties of FS and Martinotti neurons from water-exposed animals are also shown. G) Representative traces depict fundamental differences in FS (left) and Martinotti (right) neuron functional characteristics, as well as the effect of application of the HCN channel antagonist ZD-7288 (red; 50 μM).
    Figure Legend Snippet: A) Representative slice showing mDlx-GFP viral vector expression in the prelimbic mPFC. Co-labeling of mDlx-GFP and GAD67 demonstrates selectivity of the mDlx enhancer element for interneurons, as previously observed [20]. B) Simplified cartoon of cortical cell architecture, demonstrating approximate innervation profiles of deep-layer Fast-Spiking (FS; green) and Martinotti (MART; dark blue) interneurons. X94 cells (light blue) are somatostatin-positive cells generally located in Layer IV, and neurogliaform cells (NGF; yellow), as well as VIP-positive cells, are found largely in the superficial layers. C) For the present study, we confined our analyses to prototypical FS and Martinotti cells according to criteria outlined in the methods. Proportions of these cells were approximately equal in males and females. D-E) These neuron subtypes were readily discriminable by both spiking and input resistance. F) Single cell real-time qPCR revealed heterogeneity of Parv, Som, and Hcn1 markers in both FS and Martinotti cells, and thus did not prove especially valuable for subtype discrimination. Passive membrane properties of FS and Martinotti neurons from water-exposed animals are also shown. G) Representative traces depict fundamental differences in FS (left) and Martinotti (right) neuron functional characteristics, as well as the effect of application of the HCN channel antagonist ZD-7288 (red; 50 μM).

    Techniques Used: Plasmid Preparation, Expressing, Labeling, Membrane, Functional Assay



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    mdlx enhancer element  (Addgene inc)
    95
    Addgene inc mdlx enhancer element
    A) Representative slice <t>showing</t> <t>mDlx-GFP</t> viral vector expression in the prelimbic mPFC. Co-labeling of mDlx-GFP and GAD67 demonstrates selectivity of the mDlx enhancer element for interneurons, as previously observed [20]. B) Simplified cartoon of cortical cell architecture, demonstrating approximate innervation profiles of deep-layer Fast-Spiking (FS; green) and Martinotti (MART; dark blue) interneurons. X94 cells (light blue) are somatostatin-positive cells generally located in Layer IV, and neurogliaform cells (NGF; yellow), as well as VIP-positive cells, are found largely in the superficial layers. C) For the present study, we confined our analyses to prototypical FS and Martinotti cells according to criteria outlined in the methods. Proportions of these cells were approximately equal in males and females. D-E) These neuron subtypes were readily discriminable by both spiking and input resistance. F) Single cell real-time qPCR revealed heterogeneity of Parv, Som, and Hcn1 markers in both FS and Martinotti cells, and thus did not prove especially valuable for subtype discrimination. Passive membrane properties of FS and Martinotti neurons from water-exposed animals are also shown. G) Representative traces depict fundamental differences in FS (left) and Martinotti (right) neuron functional characteristics, as well as the effect of application of the HCN channel antagonist ZD-7288 (red; 50 μM).
    Mdlx Enhancer Element, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mdlx enhancer element/product/Addgene inc
    Average 95 stars, based on 1 article reviews
    mdlx enhancer element - by Bioz Stars, 2026-04
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    A) Representative slice showing mDlx-GFP viral vector expression in the prelimbic mPFC. Co-labeling of mDlx-GFP and GAD67 demonstrates selectivity of the mDlx enhancer element for interneurons, as previously observed [20]. B) Simplified cartoon of cortical cell architecture, demonstrating approximate innervation profiles of deep-layer Fast-Spiking (FS; green) and Martinotti (MART; dark blue) interneurons. X94 cells (light blue) are somatostatin-positive cells generally located in Layer IV, and neurogliaform cells (NGF; yellow), as well as VIP-positive cells, are found largely in the superficial layers. C) For the present study, we confined our analyses to prototypical FS and Martinotti cells according to criteria outlined in the methods. Proportions of these cells were approximately equal in males and females. D-E) These neuron subtypes were readily discriminable by both spiking and input resistance. F) Single cell real-time qPCR revealed heterogeneity of Parv, Som, and Hcn1 markers in both FS and Martinotti cells, and thus did not prove especially valuable for subtype discrimination. Passive membrane properties of FS and Martinotti neurons from water-exposed animals are also shown. G) Representative traces depict fundamental differences in FS (left) and Martinotti (right) neuron functional characteristics, as well as the effect of application of the HCN channel antagonist ZD-7288 (red; 50 μM).

    Journal: Neuropharmacology

    Article Title: Chronic Ethanol Exposure Alters Prelimbic Prefrontal Cortical Fast-Spiking and Martinotti Interneuron Function With Differential Sex Specificity in Rat Brain

    doi: 10.1016/j.neuropharm.2019.107805

    Figure Lengend Snippet: A) Representative slice showing mDlx-GFP viral vector expression in the prelimbic mPFC. Co-labeling of mDlx-GFP and GAD67 demonstrates selectivity of the mDlx enhancer element for interneurons, as previously observed [20]. B) Simplified cartoon of cortical cell architecture, demonstrating approximate innervation profiles of deep-layer Fast-Spiking (FS; green) and Martinotti (MART; dark blue) interneurons. X94 cells (light blue) are somatostatin-positive cells generally located in Layer IV, and neurogliaform cells (NGF; yellow), as well as VIP-positive cells, are found largely in the superficial layers. C) For the present study, we confined our analyses to prototypical FS and Martinotti cells according to criteria outlined in the methods. Proportions of these cells were approximately equal in males and females. D-E) These neuron subtypes were readily discriminable by both spiking and input resistance. F) Single cell real-time qPCR revealed heterogeneity of Parv, Som, and Hcn1 markers in both FS and Martinotti cells, and thus did not prove especially valuable for subtype discrimination. Passive membrane properties of FS and Martinotti neurons from water-exposed animals are also shown. G) Representative traces depict fundamental differences in FS (left) and Martinotti (right) neuron functional characteristics, as well as the effect of application of the HCN channel antagonist ZD-7288 (red; 50 μM).

    Article Snippet: Animals were anaesthetized using isoflurane and underwent stereotaxic surgery for bilateral implantation of an adeno-associated viral vector (AAV5) containing GFP under the control of the mDlx enhancer element (pAAV-mDlx-GFP-Fishell-1 was a gift from Gordon Fishell, Addgene plasmid # 83900; plasmid packaged into AAV5 by the UNC Vector Core).

    Techniques: Plasmid Preparation, Expressing, Labeling, Membrane, Functional Assay